Apme
2015
The Effect of a Whey Protein Supplement on Bone Mass in Older Caucasian Adults
Jane E. Kerstetter,* Jessica D. Bihuniak,* Jennifer Brindisi, Rebecca R. Sullivan, Kelsey M. Mangano, Sarah Larocque, Belinda M. Kotler, Christine A. Simpson, Anna Maria Cusano, Erin Gaffney-Stomberg, Alison Kleppinger, Jesse Reynolds, James Dziura, Anne M. Kenny,* and Karl L. Insogna*
Context: It has been assumed that the increase in urine calcium (Ca) that accompanies an increase
in dietary protein was due to increased bone resorption. However, studies using stable Ca isotopes
have found that dietary protein increases Ca absorption without increasing bone resorption.
Objective: The objective of the study was to investigate the impact of a moderately high protein
diet on bone mineral density (BMD).
Design: This was a randomized, double-blind, placebo-controlled trial of protein supplementation
daily for 18 months.
Setting: The study was conducted at two institutional research centers.
Participants: Two hundred eight older women and men with a body mass index between 19 and
32 kg/m2 and a self-reported protein intake between 0.6 and 1.0 g/kg participated in the study.
Intervention: Subjects were asked to incorporate either a 45-g whey protein or isocaloric maltodextrin
supplement into their usual diet for 18 months.
Main Outcome Measure: BMD by dual-energy x-ray absorptiometry, body composition, and markers
of skeletal and mineral metabolism were measured at baseline and at 9 and 18 months.
Results: There werenosignificant differences between groups for changes in L-spineBMD(primary
outcome) or the other skeletal sites of interest. Truncal lean mass was significantly higher in the
protein group at 18 months (P .048). C-terminal telopeptide (P .0414), IGF-1 (P .0054), and
urinary urea (P.001) were also higher in the protein group at the end of the study period. There
was no difference in estimated glomerular filtration rate at 18 months.